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Detection Image Detection method details

Name:

 QL-ELE-Cry1F-F2/Cry1Fr-n2

Description:

 Qualitative real-time PCR (TaqMan) method for detection of the cry1F gene (Scholtens et al., 2013, with minor modifications in forward primer and probe sequence)

Comment:

 Target is the cry1F gene from bacterium Bacillus thuringiensis var. aizawai.

Validation:

 in-house validation

Standardisation:

 none

Related Methods:

Type:

 element-specific

Target DNA element:
CS-cry1F_tr-BACTA

  • Oligonucleotides:

  • Forward Primer

  • Name:

    Cry1F-F2

  • Sequence:

    GACGTGGATCTTCATCTGCAATC

  • Size:

    23

  • Reverse Primer

  • Name:

    Cry1Fr-n2

  • Sequence:

    GCAACACGGCTGGCAATCG

  • Size:

    19

  • Probe

  • Name:

    Cry1F-P2

  • Sequence:

    CGCCCCCGGGATTGAAGACCCCGTAAC

  • Size:

    27

  • Amplicon:

  • Sequence:

    GACGTGGATCTTCATCTGCAATCCAGATGGCGCCCCCGGGATTGAAGACCCCGTAACTAGGAAAGTTGATACGATTGCCAGCCGTGTTGC

  • Size:

    90

Documents

Citation Type Local copy
Scholtens et al. (2012)