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Detection Image Detection method details

Name:

 QL-CON-gat/T-pinII-F/gat/T-pinII-R

Description:

 Qualitative real-time PCR (TaqMan) method for detection of the junction between the gat gene and pinII terminator (Prins et al., 2016)

Comment:

 Target is the junction between between the Bacillus licheniformis glyphosate-N-acetyltransferase (CS-gat- BACLI) gene and the proteinase inhibitor II (T-pinII-SOLTU) terminator from Solanum tuberosum.

Amplicon represented by maize 98140 (GenBank KP784699) is 112 nt instead of the published 111 nt.

Validation:

 in-house validation

Standardisation:

 national standard

Related Methods:

Type:

 construct-specific

Target GMO names:
61061, 73496, 98140, DP356043

  • Oligonucleotides:

  • Forward Primer

  • Name:

    gat/T-pinII-F

  • Sequence:

    GGACCTCACATCCTGATGTATAA

  • Size:

    23

  • Reverse Primer

  • Name:

    gat/T-pinII-R

  • Sequence:

    GCATCCTTTTATTTCATACATTA

  • Size:

    23

  • Probe

  • Name:

    gat/T-pinII-P

  • Sequence:

    CCATCTTCTGGATTGGCCAACTTAA

  • Size:

    25

  • Amplicon:

  • Sequence:

    GGACCTCACATCCTGATGTATAAGAGGATCACATAACTAGCTAGTCAGTTAACCTAGACTTGTCCATCTTCTGGATTGGCCAACTTAATTAATGTATGAAATAAAAGGATGC

  • Size:

    112

Documents

Citation Type Local copy
Prins, T.W., van Hoof, R.A., Scholtens, I.M.J., Kok, E.J. (2016). Novel TaqMan PCR screening methods for element cry3A and construct gat/T‑pinII to support detection of both known and unknown GMOs. Eur Food Res Technol, 243, 481–488.Link to the document url. peer-reviewed article Download PDF pdf