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Detection method details
Name: |
QL-ELE-00-011 |
Description: |
Qualitative real-time PCR (TaqMan) method for detection of nopaline synthase terminator (ISO 21569 AMD1). |
Comment: |
Target is the nopaline synthase terminator (T-nos) from Agrobacterium tumefaciens. |
Validation: |
ring trial validation |
Standardisation: |
ISO standard |
Related Methods: |
Type: |
element-specific |
Target DNA element: |
T-nos-RHIRD
|
-
Oligonucleotides:
Forward Primer
Name:
180-FSequence:
CATGTAATGCATGACGTTATTTATGSize:
25
Reverse Primer
Name:
180-RSequence:
TTGTTTTCTATCGCGTATTAAATGTSize:
25
Probe
Name:
Tm-180Sequence:
ATGGGTTTTTATGATTAGAGTCCCGCAASize:
28
-
Amplicon:
Sequence:
catgtaatgcatgacgttatttatgagatgggtttttatgattagagtcccgcaattatacatttaatacgcgatagaaaacaaSize:
84
Documents
Citation | Type | Local copy |
---|---|---|
Reiting R., Broll H., Waiblinger H.-U., Grohmann L.; Collaborative Study of a T-nos Real-Time PCR Method for Screening of Genetically Modified Organisms in Food Products J. Verbr. Lebensm. 2:116-121 (2007)![]() |
peer-reviewed article | |
EU-RL GMFF GMOMETHODS database; QL-ELE-00-011![]() |
EU reference method protocol |