Detection method details
Name: |
QT-EVE-ZM-006 |
Description: |
Quantitative real-time PCR (TaqMan) method for detection of maize event Bt11 (ISO 21570) |
Comment: |
target is the 3' integration border region (IBR) between the insert of maize event Bt11 and the maize host genome |
Validation: |
ring trial validation |
Standardisation: |
ISO standard |
Related Methods: |
Type: |
event-specific |
Target GMO name: |
Bt11 |
-
Oligonucleotides:
Forward Primer
Name:
Bt113JForSequence:
GCGGAACCCCTATTTGTTTASize:
20
Reverse Primer
Name:
Bt113JRevSequence:
TCCAAGAATCCCTCCATGAGSize:
20
Probe
Name:
Bt113JFTSequence:
AAATACATTCAAATATGTATCCGCTCASize:
27
-
Amplicon:
Sequence:
gcggaacccctatttgtttannnnnnnaaatacattcaaatatgtatccgctcatggagggattcttggaSize:
70
Documents
Citation | Type | Local copy |
---|---|---|
EU-RL GMFF GMOMETHODS database; QT-EVE-ZM-006 | EU reference method protocol | |
Foodstuffs - Methods of analysis for the detection of genetically modified organisms and derived products - Quantitative nucleic acid based methods ISO 21570:1-103 (2005) | other document |