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Detection Image Detection method details

Name:

 QL-TAX-GM-001

Description:

 Qualitative real-time PCR (SYBRGreen) method for detection of soybean lectin gene (Barbau-Piednoir et al., 2014)

Comment:

 Target is the endogenous lectin (Le1) gene of Glycine max

Validation:

 ring trial validation

Standardisation:

 unknown

Related Methods:

Type:

 taxon-specific

Target Species name:

 Glycine max

  • Oligonucleotides:

  • Forward Primer

  • Name:

    sltm 1

  • Sequence:

    AACCGGTAGCGTTGCCAG

  • Size:

    18

  • Reverse Primer

  • Name:

    sltm 2

  • Sequence:

    AGCCCATCTGCAAGCCTTT

  • Size:

    19

  • Amplicon:

  • Sequence:

    aaccggtagcgttgccagnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnaaaggcttgcagatgggct

  • Size:

    81

Documents

Citation Type Local copy
Barbau-Piednoir, E., Stragier, P., Roosens, N., Mazzara, M., Savini, C., Van den Eede, G., Van den Bulcke, M. (2014). Inter-laboratory testing of GMO detection by combinatory SYBR®Green PCR screening (CoSYPS). Food Analytical Methods. 7, 1719-1728.Link to the document url. peer-reviewed article
EU-RL GMFF GMOMETHODS database; QL-TAX-GM-001Link to the document url. EU reference method protocol