Detection method details
Name: |
QT-EVE-HF-1F/HF-2R |
Description: |
Quantitative real-time PCR (SYBRGreen) method for detection of tomato event Huafan No 1 (Yang et al., 2013) |
Comment: |
Target is the 3' integration border region (IBR) between the insert of tomato event Huafan No 1 and the tomato host genome.
The nucleotide sequence of 1639 bp used for the development of this event-specific PCR detection method is given in GenBank EU363226. |
Validation: |
in-house validation |
Standardisation: |
unknown |
Related Methods: |
QT-TAX-SL-002 |
Type: |
event-specific |
Target GMO name: |
Huafan No 1 |
-
Oligonucleotides:
Forward Primer
Name:
HF-1FSequence:
CTGTTGCCCGTCTCACTGSize:
18
Reverse Primer
Name:
HF-2RSequence:
AATCGTGTATGACCTTTTAGSize:
20
-
Amplicon:
Sequence:
ctgttgcccgtctcactggtgaaaagaaaaaccaccccagtacattaaaaacgtccgcaatgtgttattaagttaaatatataatgctattttatatattgttttaagttaaatatataataatattctccaaatggacatttaacttgactttttttatctattaaaattacttttcataacaattaaattataaacttgctaattcctaaaaggtcatacacgattSize:
228
Documents
| Citation | Type | Local copy |
|---|---|---|
Yang, C., Zhang, D., Yang, L. (2013) Development of event-specific PCR detection methods for genetically modified tomato Huafan No. 1. J Sci Food Agric, 93(3), 652-60 |
peer-reviewed article |