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Detection Image Detection method details

Name:

 QT-EVE-GM-017

Description:

 Quantitative real-time PCR (TaqMan) method for detection of soybean event SYHT0H2(EU-RL GMFF GMOMETHODS database as of September 19, 2016)

Comment:

 Target is the 3' integration border region (IBR) between the insert of soybean event SYHT0H2 and the soybean host genome

Validation:

 ring trial validation

Standardisation:

 EU reference method

Related Methods:

 QT-TAX-GM-002

Type:

 event-specific

Target GMO name:

 SYHT0H2

  • Oligonucleotides:

  • Forward Primer

  • Name:

    FE08316-F

  • Sequence:

    GGGAATTGGGTACCATGCC

  • Size:

    19

  • Reverse Primer

  • Name:

    FE08317-R

  • Sequence:

    TGTGTGCCATTGGTTTAGGGT

  • Size:

    21

  • Probe

  • Name:

    FE08318-P

  • Sequence:

    CCAGCATGGCCGTATCCGCAA

  • Size:

    21

  • Amplicon:

  • Sequence:

    gggaattgggtaccatgccnnnnnnnccagcatggccgtatccgcaannnnnnnnnnnnnnnnnnnnaccctaaaccaatggcacaca

  • Size:

    88

Documents

Citation Type Local copy
EU-RL GMFF GMOMETHODS database; QT-EVE-GM-017Link to the document url. EU reference method protocol