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Detection Image Detection method details

Name:

 QT-EVE-BN-006

Description:

 Quantitative real-time PCR (TaqMan) method for detection of oilseed rape event Rf1 (EU-RL GMFF GMOMETHODS database as of October 01, 2014)

Comment:

 Target is the 5' integration border region (IBR) between the insert of oilseed rape event Rf1 and the oilseed rape host genome

Validation:

 in-house validation

Standardisation:

 EU reference method

Related Methods:

 QT-TAX-BN-012

Type:

 event-specific

Target GMO name:

 Rf1

  • Oligonucleotides:

  • Forward Primer

  • Name:

    MDB118

  • Sequence:

    CTAAGGGAGGTCAAGATGTAGC

  • Size:

    22

  • Reverse Primer

  • Name:

    KVM170

  • Sequence:

    CGGGCCTAACTTTTGGTGTG

  • Size:

    20

  • Probe

  • Name:

    TM022

  • Sequence:

    CTCATCATCCTCACCCAGTCAGCATCA

  • Size:

    27

  • Amplicon:

  • Sequence:

    ctaagggaggtcaagatgtagcnnnnnnnnaacccttttccatcttttccggtgactacannnnnctcatcatcctcacccagtcagcatcancacaccaaaagttaggcccg

  • Size:

    113

Documents

Citation Type Local copy
EU-RL GMFF GMOMETHODS database; QT-EVE-BN-006Link to the document url. EU reference method protocol