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Detection Image Detection method details

Name:

 QL-TAX-Acc1-F/Acc1-R

Description:

 Quanlitative real-time PCR (TaqMan) method for detection of alfalfa (Guertler et al. 2018).

Comment:

 Target is the endogenous acetyl CoA carboxylase (acc1) of Medicago sativa.

Validation:

 ring trial validation

Standardisation:

 national standard

Related Methods:

Type:

 taxon-specific

Target Species name:

 Medicago sativa

  • Oligonucleotides:

  • Forward Primer

  • Name:

    Acc1-F

  • Sequence:

    GATCAGTGAACTTCGCAAAGTAC

  • Size:

    23

  • Reverse Primer

  • Name:

    Acc1-R

  • Sequence:

    CAACGACGTGAACACTACAAC

  • Size:

    21

  • Probe

  • Name:

    Acc1-P

  • Sequence:

    TGAATGCTCCTGTGATCTGCCCATGC

  • Size:

    26

  • Amplicon:

  • Sequence:

    GATCAGTGAACTTCGCAAAGTACTCGGTTAGTAGACAGTGAATGCTCCTGTGATCTGCCCATGCACTCATGTTGTAGTGTTCACGTCGTTG

  • Size:

    91

Documents

Citation Type Local copy
Guertler et al., Development of event-specific qPCR detection methods for genetically modified alfalfa events J101, J163 and KK179. Biomolecular Detection and Quantification 2019, 17, 100076.Link to the document url. peer-reviewed article