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Detection Image Detection method details

Name:

 QL-EVE-CP-001

Description:

 Qualitative conventional PCR method for the detection of papaya event Huanong No. 1 (EU-RL GMFF GMOMETHODS database as of September 19, 2016)

Comment:

 Target is the 5' integration border region (IBR) between the insert of papaya event Huanong No. 1 and the papaya host genome

Validation:

 ring trial validation

Standardisation:

 EU reference method

Related Methods:

Type:

 event-specific

Target GMO name:

 Huanong No. 1

  • Oligonucleotides:

  • Forward Primer

  • Name:

    HN-F

  • Sequence:

    ATTGACGAGTACAAGGAGACGC

  • Size:

    22

  • Reverse Primer

  • Name:

    HN-R

  • Sequence:

    CTCCGCTCATGATCAGATTGT

  • Size:

    21

  • Probe

  • Name:

  • Sequence:

  • Size:

  • Amplicon:

  • Sequence:

    attgacgagtacaaggagacgccttttaatttgtttaaattaaataaaaacataaattattaagcgacgtaataataaacacgtcataaattttttcaaaaaataaaatttcttttattttctaaaaagtagttgattcgcccaatagcagccagtcccttcccgcttcagtgacaacgtcgagcacagctgcgcaaggaacgcccgtcgtggccagccacgatagcctcaaacactgatagtttaaactgaaggcgggaaacgacaatctgatcatgagcggag

  • Size:

    285

Documents

Citation Type Local copy
Wei, J. et al. (2016). Collaborative trial for the validation of event-specific PCR detection methods of genetically modified papaya Huanong No. 1. Food Chemistry, 194, 20-25.Link to the document url. peer-reviewed article
EU-RL GMFF GMOMETHODS database; QL-EVE-CP-001Link to the document url. EU reference method protocol