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Detection Image Detection method details

Name:

 QL-EVE-558F/558R

Description:

 Qualitative real-time PCR (TaqMan) method for detection of Bacillus subtilis isolate e871 (Paracchini et al., 2017).

Comment:

 Target is the integration site between the inserted cat gene and the endogenous recA gene of Bacillus subtilis.

Validation:

 unknown

Standardisation:

 none

Related Methods:

Type:

 event-specific

Target GMO name:

 e871 Bacillus subtilis

  • Oligonucleotides:

  • Forward Primer

  • Name:

    558-F

  • Sequence:

    CGAGCTTTTGCGCGTATA

  • Size:

    18

  • Reverse Primer

  • Name:

    558-R

  • Sequence:

    GCCATTCCAATACAAAACCACATA

  • Size:

    24

  • Probe

  • Name:

    558-Tm

  • Sequence:

    CGGATCTAACGCATGCTCCGCA

  • Size:

    22

  • Amplicon:

  • Sequence:

    cgagcttttgcgcgtataccggatctaacgcatgctccgcatcgattataggtatgtggttttgtattggaatggc

  • Size:

    76

Documents

Citation Type Local copy