Detection method details
Name: |
QT-EVE-BN-007 |
Description: |
Quantitative real-time PCR (TaqMan) method for detection of oilseed rape event Rf2 (verified by the EU-RL GMFF in the context of Commission Decision 2007/306/EC) |
Comment: |
Target is the 5'integration border region (IBR) between the insert of oilseed rape event RF2 and the oilseed rape host genome |
Validation: |
in-house validation |
Standardisation: |
unknown |
Related Methods: |
QT-TAX-BN-012 |
Type: |
event-specific |
Target GMO name: |
Rf2 |
-
Oligonucleotides:
Forward Primer
Name:
MDB207Sequence:
GGGTGAGACAATATATCGACGSize:
21
Reverse Primer
Name:
KVM171Sequence:
GGGCATCGCACCGGTGAGSize:
18
Probe
Name:
TM024Sequence:
CACCGGCCAAATTCGCTCTTAGCCGTSize:
26
-
Amplicon:
Sequence:
gggtgagacaatatatcgacgnnnnnnnnnnnnnnnnnnnnnnnnnnnnnncaccggccaaattcgctcttagccgtnnnnnnnnnctcaccggtgcgatgcccSize:
104
Documents
Citation | Type | Local copy |
---|---|---|
EU-RL GMFF GMOMETHODS database; QT-EVE-BN-007 | EU reference method protocol |