Detection method details
Name: |
QL-CON-PGS-bar-A2/PGS-bar-B2 |
Description: |
Qualitative conventional PCR method for detection of the junction between P-SSU promoter and the bar gene (BVL G30.40-13). |
Comment: |
Target is the junction region between the small subunit promoter (P-SSU) from Arabidopsis thaliana and the phosphinothricin acetyltransferase gene (bar) from Streptomyces hygroscopicus (BVL G30.40-13). |
Validation: |
ring trial validation |
Standardisation: |
national standard |
Related Methods: |
Type: |
construct-specific |
Target GMO names: |
Ms1, Ms11, Ms8, RM3-3, RM3-4, RM3-6, Rf1, Rf2, Rf3, Rf3 (Brassica juncea)
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Oligonucleotides:
Forward Primer
Name:
PGS-bar-A2Sequence:
GAAGTTGACCGTGCTTGTCTSize:
20
Reverse Primer
Name:
PGS-bar-B2Sequence:
CAAGTCCACCAGGCAAGTAASize:
20
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Amplicon:
unknown