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Detection Image Detection method details

Name:

 QL-ELE-00-025

Description:

 Qualitative real-time PCR (TaqMan) method for detection of the synthetic phosphinothricin acetyltransferase (pat) gene (BVL L00.00-154). EU-RL GMFF GMOMETHODS database as of December 23, 2016.

Comment:

 Target is the synthetic phosphinothricin acetyltransferase (pat) gene from bacterium Streptomyces viridochromogenes.

For details of the detection of bar (partim bar) when applied as duplex real-time PCR method refer to QL-ELE-00-026

Validation:

 ring trial validation

Standardisation:

 EU reference method

Related Methods:

Type:

 element-specific

Target DNA element:
CS-pat-STRVR

  • Oligonucleotides:

  • Forward Primer

  • Name:

    pat-F

  • Sequence:

    CGCGGTTTGTGATATCGTTAAC

  • Size:

    22

  • Reverse Primer

  • Name:

    pat-R

  • Sequence:

    TCTTGCAACCTCTCTAGATCATCAA

  • Size:

    25

  • Probe

  • Name:

    pat-P

  • Sequence:

    AGGACAGAGCCACAAACACCACAAGAGTG

  • Size:

    29

  • Amplicon:

  • Sequence:

    cgcggtttgtgatatcgttaaccattacattgagacgtctacagtgaactttaggacagagccacaaacaccacaagagtggattgatgatctagagaggttgcaaga

  • Size:

    108

Documents

Citation Type Local copy
Huber, I. et al. (2013). Development and Validation of Duplex, Triplex, and Pentaplex Real-Time PCR Screening Assays for the Detection of Genetically Modified Organisms in Food and Feed. J. Agric. Food Chem. 61, 10293–10301Link to the document url. peer-reviewed article
EU-RL GMFF GMOMETHODS database; QL-ELE-00-025Link to the document url. EU reference method protocol
Debode F., Huber I., Macarthur R., et al. (2016) Inter-laboratory studies for the validation of two singleplex (tE9 and pea lectin) and one duplex (pat/bar) real-time PCR methods for GMO detection. Food Control, 73, 452-461Link to the document url. peer-reviewed article