Detection method details
Name: |
QL-EVE-MDB110/SHA016 |
Description: |
Qualitative real-time PCR (TaqMan) method for detection of of oilseed rape event OXY-235 (EURL GMFF, 2016)) |
Comment: |
Target is the 3' integration border region (IBR) between the insert of Brassica napus event OXY-235 and the Brassica napus host genome |
Validation: |
in-house validation |
Standardisation: |
EU reference method |
Related Methods: |
QT-TAX-BN-012 |
Type: |
event-specific |
Target GMO name: |
OXY-235 |
-
Oligonucleotides:
Forward Primer
Name:
MDB110Sequence:
CGAATTTGGCCTGTAGACCTCAATTGCGAGCSize:
31
Reverse Primer
Name:
SHA016Sequence:
CGCCGTGCTTCATAACCGSize:
18
Probe
Name:
TM033Sequence:
CAGCATCATCACACCAAAAGTTAGGCCSize:
27
-
Amplicon:
Sequence:
CGAATTTGGCCTGTAGACCTCAATTGCGAGCTTTCTAATTTCAAACTATTCGGGCCTAACTTTTGGTGTGATGATGCTGACTGGCAAGTTAATCTAGTTTCCGGTTATGAAGCACGGCGSize:
119
Documents
| Citation | Type | Local copy |
|---|---|---|
Event-Specific Method for the Detection of Oilseed Rape Oxy-235 Using Real-Time PCR. EURL-GMFF Validation Report (2016) |
other document |