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Detection Image Detection method details

Name:

 QT-TAX-GH-016

Description:

 Quantitative PCR method for detection of putative cotton SAH7 protein gene (123 bp) (Mazzara et al., 2006).

Estimated number of target copies per haploid genome (Jacchia et al. 2018): 2

Comment:

 Target is the IVS of the putative Sinapis Arabidopsis Homolog 7 (SAH7) protein gene of Gossypium hirsutum D-subgenome.

The assay for AdhC should be preferred for GMO quantitative analysis, but SAH7 can also be used because primers and probe anneal without mismatches to both target DNAs.

Validation:

 ring trial validation

Standardisation:

 EU reference method

Related Methods:

Type:

 taxon-specific

Target Species name:

 Gossypium hirsutum

  • Oligonucleotides:

  • Forward Primer

  • Name:

    Sah7-uni-f1

  • Sequence:

    AGTTTGTAGGTTTTGATGTTACATTGAG

  • Size:

    28

  • Reverse Primer

  • Name:

    Sah7-uni-r1

  • Sequence:

    GCATCTTTGAACCGCCTACTG

  • Size:

    21

  • Probe

  • Name:

    Sah7-uni-s1

  • Sequence:

    AAACATAAAATAATGGGAACAACCATGACATGT

  • Size:

    33

  • Amplicon:

  • Sequence:

    agtttgtaggttttgatgttacattgagnnnnnnnnnnnnnnnnnnnnnnnaaacataaaataatgggaacaaccatgacatgtnnnnnnnnnnnnnnnnnncagtaggcggttcaaagatgc

  • Size:

    123

Documents

Citation Type Local copy
Jacchia S., Kagkli D.F., Lievens A., Angers-Loustau A., Savini C., Emons H., Mazzara M. (2018) Identification of single target taxon-specific reference assays for the most commonly genetically transformed crops using digital droplet PCR. Food Control 93,191-200Link to the document url. peer-reviewed article
EU-RL GMFF GMOMETHODS database; QT-TAX-GH-016Link to the document url. EU reference method protocol