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Detection Image Detection method details

Name:

 QT-EVE-GM-003

Description:

 Quantitative real-time PCR (TaqMan) method for detection of soybean event MON87705 (EU-RL GMFF GMOMETHODS database as of July 21, 2014)

Comment:

 Target is the 3' integration border region (IBR) between the insert of soybean event MON87705 and the soybean host genome

Validation:

 ring trial validation

Standardisation:

 EU reference method

Related Methods:

 QT-TAX-GM-002

Type:

 event-specific

Target GMO name:

 MON87705

  • Oligonucleotides:

  • Forward Primer

  • Name:

    MON 87705 primer 1

  • Sequence:

    TTCCCGGACATGAAGCCATTTAC

  • Size:

    23

  • Reverse Primer

  • Name:

    MON 87705 primer 2

  • Sequence:

    ACAACGGTGCCTTGGCCCAAAG

  • Size:

    22

  • Probe

  • Name:

    MON 87705 probe

  • Sequence:

    AAGAGACTCAGGGTGTTGTTATCACTGCGG

  • Size:

    30

  • Amplicon:

  • Sequence:

    ttcccggacatgaagccatttacnnnnnaagagactcagggtgttgttatcactgcggnnnnnnctttgggccaaggcaccgttgt

  • Size:

    86

Documents

Citation Type Local copy
EU-RL GMFF GMOMETHODS database; QT-EVE-GM-003Link to the document url. EU reference method protocol