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Detection Image Detection method details

Name:

 QT-EVE-GH-012

Description:

 Quantitative real-time PCR (TaqMan) method for detection of cotton event COT102 (EU-RL GMFF GMOMETHODS database as of February 18, 2020)

Comment:

 Target is the 3' integration border region (IBR) between the insert of cotton event COT102 and the cotton host genome

Validation:

 ring trial validation

Standardisation:

 EU reference method

Related Methods:

 QT-TAX-GH-021

Type:

 event-specific

Target GMO name:

 COT102

  • Oligonucleotides:

  • Forward Primer

  • Name:

    COT102_3_89F

  • Sequence:

    TCTCCGCTCATGATCAGATTGTC

  • Size:

    23

  • Reverse Primer

  • Name:

    COT102_3_181R

  • Sequence:

    CAGTAACAGTACAGTCGGTGTAGGG

  • Size:

    25

  • Probe

  • Name:

    COT102_3_115T

  • Sequence:

    TCCCGCCTTCAGTTTAAACTATCAGTGTTTAAT

  • Size:

    33

  • Amplicon:

  • Sequence:

    TCTCCGCTCATGATCAGATTGTCGTTTCCCGCCTTCAGTTTAAACTATCAGTGTTTAATAAATATGGGCAATCTTTCCCTACACCGACTGTACTGTTACTG

  • Size:

    101

Documents

Citation Type Local copy
EU-RL GMFF GMOMETHODS database; QT-EVE-GH-012Link to the document url. EU reference method protocol