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Detection Image Detection method details

Name:

 QT-CON-00-006

Description:

 Quantitative real-time PCR (TaqMan) method for detection of maize event Bt11 (Shindo et al., 2002)

Comment:

 Target is the junction region between the Intron 6 (IVS6) from maize alcohol dehydrogenase 1 gene (adh1-1S) and a synthetic cryIA(b) gene

Validation:

 ring trial validation

Standardisation:

 ISO standard

Related Methods:

 QT-TAX-ZM-006

Type:

 construct-specific

Target GMO names:
Bt10 maize, Bt11

  • Oligonucleotides:

  • Forward Primer

  • Name:

    Bt11 3-5’

  • Sequence:

    AAAAGACCACAACAAGCCGC

  • Size:

    20

  • Reverse Primer

  • Name:

    Bt11 3-3'

  • Sequence:

    CAATGCGTTCTCCACCAAGTACT

  • Size:

    23

  • Probe

  • Name:

    Bt11-2-Taq

  • Sequence:

    CGACCATGGACAACAACCCAAACATCA

  • Size:

    27

  • Amplicon:

  • Sequence:

    aaaagaccacaacaagccgcnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnagtacttggtggagaacgcattg

  • Size:

    127

Documents

Citation Type Local copy
Shindo Y., Kuribara H., Matsuoka T., Futo S., Sawada C., Shono J., Akiyama H., Goda Y., Toyoda M., Hino A.; Validation of real-time PCR analyses for line-specific quantitation of genetically modified maize and soybean using new reference molecules J AOALink to the document url. peer-reviewed article
EU-RL GMFF GMOMETHODS database; QT-CON-00-006Link to the document url. EU reference method protocol
Foodstuffs - Methods of analysis for the detection of genetically modified organisms and derived products - Quantitative nucleic acid based methods ISO 21570:1-103 (2005)Link to the document url. other document