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Detection Image Detection method details

Name:

 QL-EVE-MS-KK179-F/KK179-R

Description:

 Qualitative real-time PCR (TaqMan) method for detection of alfalfa event KK179 (Guertler et al. 2018).

Comment:

 Target is the 5' integration border region (IBR) between the insert of alfalfa event KK179 and the alfalfa host genome.

Validation:

 ring trial validation

Standardisation:

 national standard

Related Methods:

 QL-TAX-Acc1-F/Acc1-R

Type:

 event-specific

Target GMO name:

 KK179

  • Oligonucleotides:

  • Forward Primer

  • Name:

    KK179-F

  • Sequence:

    CTTAGGGCACTTGTTAGCATTTTC

  • Size:

    24

  • Reverse Primer

  • Name:

    KK179-R

  • Sequence:

    CCATATTGACCATCATACTCATTGC

  • Size:

    25

  • Probe

  • Name:

    KK179-P

  • Sequence:

    TGGCTTCATGTCCGGGAAATCTACATGG

  • Size:

    28

  • Amplicon:

  • Sequence:

    CTTAGGGCACTTGTTAGCATTTTCCTAAAAATAATAGATACAGTTGAAATCGTATTTGAAATTAATTAAGTAGATATTTAAATCGAATCAAACCACAATTGATAATAATCTTCAATTGTAAATGGCTTCATGTCCGGGAAATCTACATGGATCAGCAATGAGTATGATGGTCAATATGG

  • Size:

    179

Documents

Citation Type Local copy
Guertler et al., Development of event-specific qPCR detection methods for genetically modified alfalfa events J101, J163 and KK179. Biomolecular Detection and Quantification 2019, 17, 100076.Link to the document url. peer-reviewed article