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Detection Image Detection method details

Name:

 QL-EVE-TA-MON71200

Description:

 Qualitative real-time PCR (TaqMan) method for detection of wheat event MON71200 (EU-RL GMFF GMOMETHODS database as of July 3, 2018).

Comment:

 Target is the 3' integration border region (IBR) between the insert of wheat event MON71200 and the wheat host genome.

Event-specific diagnostic method for the detection of MON71200 wheat (Simplex). Developed by the Genotyping – Botany Laboratory, Ottawa Plant Laboratory, Canadian Food Inspection Agency CFIA).

Validation:

 in-house validation

Standardisation:

 EU reference method

Related Methods:

Type:

 event-specific

Target GMO name:

 MON71200

  • Oligonucleotides:

  • Forward Primer

  • Name:

    MON71200-3 ’ junction-1F

  • Sequence:

    CACGACGGTCATCGAGC

  • Size:

    17

  • Reverse Primer

  • Name:

    MON71200-3 ’ junction-1R

  • Sequence:

    CCGTTCGTCATTGACTGTT

  • Size:

    19

  • Probe

  • Name:

    MON71200-3 ’ junction-P

  • Sequence:

    CATACGGAAAAGATGCTGCAGGGAATATATTGAAC

  • Size:

    35

  • Amplicon:

  • Sequence:

  • Size:

    139

Documents

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