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Detection Image Detection method details

Name:

 QL-EVE-SQ0718/SQ0719

Description:

 Qualitative real-time PCR (TaqMan) method for detection of wheat event MON71800 (verification report of the EU-RL GMFF, 4 Dec 2013).

Comment:

 Target is the region spanning the insert-to-plant junction in wheat event MON71800. The probe is labelled with a MGB-NFQ (minor groove binding non-fluorescent quencher) as quencher at its 3’ end.

Validation:

 in-house validation

Standardisation:

 none

Related Methods:

 QT-TAX-wx012-5'/wx012-3'

Type:

 event-specific

Target GMO name:

 MON71800

  • Oligonucleotides:

  • Forward Primer

  • Name:

    SQ0718

  • Sequence:

    TTCTTCTCTCTCTTTGAATCTCAATACAA

  • Size:

    29

  • Reverse Primer

  • Name:

    SQ0719

  • Sequence:

    CCCCCATTTGGACGTGAA

  • Size:

    18

  • Probe

  • Name:

    PB0101

  • Sequence:

    TCCCCCTCTCTAATTC

  • Size:

    16

  • Amplicon:

  • Sequence:

    ttcttctctctctttgaatctcaatacaannnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnttcacgtccaaatggggg

  • Size:

    95

Documents

Citation Type Local copy
Report on the Verification of the Performance of a Method for the Detection of Event MON71800 in Wheat Using Real-Time PCR (EURL-GMFF 4 Dec 2013)Link to the document url. EU reference method protocol