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Detection Image Detection method details

Name:

 QL-ELE-B'nase-F-n4/BNaseR-n3

Description:

 Qualitative real-time PCR (TaqMan) method for detection of the barnase gene (Scholtens et al. 2013).

Comment:

 Target is the barnase gene from bacterium Bacillus amyloliquefaciens.

Validation:

 in-house validation

Standardisation:

 none

Related Methods:

Type:

 element-specific

Target DNA element:
CS-barnase

  • Oligonucleotides:

  • Forward Primer

  • Name:

    B'nase-F-n4

  • Sequence:

    TGGTACCGGTTATTCAACACG

  • Size:

    21

  • Reverse Primer

  • Name:

    BNaseR-n3

  • Sequence:

    GAGGGCTTGTGCTTCTGATTTT

  • Size:

    22

  • Probe

  • Name:

    BNaseP-n3

  • Sequence:

    GTCTGAAGATAATCCGCAACCC

  • Size:

    22

  • Amplicon:

  • Sequence:

    tggtaccggttattcaacacgtttgacggggttgcggattatcttcagacatatcataagctacctgataattacattacaaaatcagaagcacaagccctc

  • Size:

    102

Documents

Citation Type Local copy
Scholtens et al. (2012)