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Detection Image Detection method details

Name:

 QL-ELE-00-002

Description:

 Qualitative conventional PCR method for detection of neomycin phosphotransferase II gene (ISO 21569)

Comment:

 Target is the neomycin phosphotransferase II (nptII) gene from Escherichia colinpt

Validation:

 ring trial validation

Standardisation:

 ISO standard

Related Methods:

Type:

 element-specific

Target DNA element:
CS-nptII-ECOLX, V-nptII-ECOLX

  • Oligonucleotides:

  • Forward Primer

  • Name:

    APH2 short

  • Sequence:

    CTCACCTTGCTCCTGCCGAGA

  • Size:

    21

  • Reverse Primer

  • Name:

    APH2 reverse

  • Sequence:

    CGCCTTGAGCCTGGCGAACAG

  • Size:

    21

  • Amplicon:

  • Sequence:

    ctcaccttgctcctgccgagannnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnctgttcgccaggctcaaggcg

  • Size:

    215

Documents

Citation Type Local copy
EU-RL GMFF GMOMETHODS database; QL-ELE-00-002Link to the document url. EU reference method protocol
Foodstuffs - Methods of analysis for the detection of genetically modified organisms and derived products - Qualitative nucleic acid based methods ISO 21569:1-69 (2005)Link to the document url. other document