Detection method details
Name: |
QL-PLN-00-007 |
Description: |
Qualitative conventional PCR method for detection of chloroplast tRNA-Leu intron (ISO/FDIS 21569) |
Comment: |
Target is the conserved chloroplast tRNA-Leu (trnL) intron. For different plant species the amplicon size varies between approx. 500-600 bp. The amplicon shown here is of a potato sample. |
Validation: |
ring trial validation |
Standardisation: |
ISO standard |
Related Methods: |
Type: |
taxon-specific |
Target Species name: |
all-plants |
-
Oligonucleotides:
Forward Primer
Name:
A1Sequence:
CGAAATCGGTAGACGCTACGSize:
20
Reverse Primer
Name:
A2Sequence:
GGGGATAGAGGGACTTGAACSize:
20
-
Amplicon:
Sequence:
cgaaatcggtagacgctacgnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnngttcaagtccctctatccccSize:
571
Documents
Citation | Type | Local copy |
---|---|---|
Foodstuffs - Methods of analysis for the detection of genetically modified organisms and derived products - Qualitative nucleic acid based methods ISO 21569:1-69 (2005) | other document | |
EU-RL GMFF GMOMETHODS database; QL-PLN-00-007 | EU reference method protocol |