Detection method details
Name: |
QT-EVE-ZM-021 |
Description: |
Quantitative real-time PCR (TaqMan) method for detection of maize event 98140 (EU-RL GMFF GMOMETHODS database as January 10, 2014) |
Comment: |
Target is the 5' integration border region (IBR) between the insert of maize event 98140 and the maize host genome |
Validation: |
ring trial validation |
Standardisation: |
EU reference method |
Related Methods: |
QT-TAX-ZM-014 |
Type: |
event-specific |
Target GMO name: |
98140 |
-
Oligonucleotides:
Forward Primer
Name:
DP098-f6Sequence:
GTGTGTATGTCTCTTTGCTTGGTCTTSize:
26
Reverse Primer
Name:
DP098-r2Sequence:
GATTGTCGTTTCCCGCCTTCSize:
20
Probe
Name:
DP098-p5Sequence:
CTCTATCGATCCCCCTCTTTGATAGTTTAAACTSize:
33
-
Amplicon:
Sequence:
gtgtgtatgtctctttgcttggtcttnctctatcgatccccctctttgatagtttaaactgaaggcgggaaacgacaatcSize:
80
Documents
Citation | Type | Local copy |
---|---|---|
Savini C., Bonfini L., Querci M., Mazzara M., Cordeil S., Van Den Eede G.; Event-specific Method for the Quantification of Maize 98140 Using Real-time PCR - Validation Report and Protocol Online Publication (2011) | EU reference method protocol | |
EU-RL GMFF GMOMETHODS database; QT-EVE-ZM-021 | EU reference method protocol |