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Detection Image Detection method details

Name:

 QL-CON-35S-F/nptII-R

Description:

 Qualitative real-time PCR (TaqMan) method for detection of the junction between the CaMV 35S promoter and the nptII gene (ISO/TS 21569-9).

Comment:

 Target is the junction region between the Cauliflower mosaic virus (CaMV) 35S promoter (P-35S) and the neomycin phophotransferase II (nptII) gene.

Validation:

 ring trial validation

Standardisation:

 ISO standard

Related Methods:

Type:

 construct-specific

Target GMO names:
23-18-17, 23-198, 31807, 31808, 5345, 8338, ATBT04-06, ATBT04-27, ATBT04-30, ATBT04-31, ATBT04-36, BT10 potato, BT12 potato, BT16 potato, BT17 potato, BT18 potato, BT23 potato, BT6 potato, BXN10211, CZW-3, EE-1, Event 1, FLAVR SAVR, MON1076, MON1445, MON15985, MON1698, MON531, MON757, MON80100, MON863, MON87460, SP951, SPBT02-7, pap1 GM Gypsophila

  • Oligonucleotides:

  • Forward Primer

  • Name:

    35S-F

  • Sequence:

    TATCCTTCGCAAGACCCTTCC

  • Size:

    21

  • Reverse Primer

  • Name:

    nptII-R

  • Sequence:

    GATTGTCTGTTGTGCCCAGTCA

  • Size:

    22

  • Probe

  • Name:

    nptII-Tm2

  • Sequence:

    AGCCGAATAGCCTCTCCACCCAAGC

  • Size:

    25

  • Amplicon:

  • Sequence:

    tatccttcgcaagacccttcctctatataaggaagttcatttcatttggagaggacacgctgaaatcaccagtctctctctacaagatcggggatctctagctagacgatcgtttcgcatgattgaacaagatggattgcacgcaggttctccggccgcttgggtggagaggctattcggctatgactgggcacaacagacaatc

  • Size:

    205

Documents

Citation Type Local copy
Reiting, R. (2010). Real-time PCR methods for the detection of DNA constructs with the nptII gene for the detection of genetically modified plants in food, feed and seed. J. Verbr. Lebensm. 5, 377-390.Link to the document url. peer-reviewed article
Horizontal methods for molecular biomarker analysis — Methods of analysis for the detection of genetically modified organisms and derived productsPart 9: Construct-specific real-time PCR based screening method for the detection of the P35S-nptII DNA-sequence (ISO/TS 21569-9)Link to the document url. other document