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Detection Image Detection method details

Name:

 QT-EVE-ZM-027

Description:

 Quantitative real-time PCR (TaqMan) method for detection of MZHG0JG. (EURL GMFF GMOMETHODS database as of 2018).

Comment:

 Target is the 3' integration border region (IBR) between the insert of maize event MZHG0JG and the maize host genome.

Validation:

 ring trial validation

Standardisation:

 EU reference method

Related Methods:

 QT-TAX-ZM-014

Type:

 event-specific

Target GMO name:

 MZHG0JG

  • Oligonucleotides:

  • Forward Primer

  • Name:

    MZHG0JG forward primer

  • Sequence:

    CAACTAGCTAGATTAATTAACGCAATCTG

  • Size:

    29

  • Reverse Primer

  • Name:

    MZHG0JG reverse primer

  • Sequence:

    ATTTGTTTGCAAGGTGTGGGA

  • Size:

    21

  • Probe

  • Name:

    MZHG0JG probe

  • Sequence:

    TTAAGTTGTCTAAGCGTCAATTTG

  • Size:

    24

  • Amplicon:

  • Sequence:

    caactagctagattaattaacgcaatctgnnnttaagttgtctaagcgtcaatttgnnnntcccacaccttgcaaacaaat

  • Size:

    81

Documents

Citation Type Local copy
EU-RL GMFF GMOMETHODS database; QT-EVE-ZM-027Link to the document url. EU reference method protocol