Detection method details
Name: |
QT-EVE-GH-004 |
Description: |
Quantitative real-time PCR (TaqMan) method for detection of cotton event MON531 (EU-RL GMFF GMOMETHODS database as of January 13, 2014) |
Comment: |
Target is the 5' integration border region (IBR) between the insert of cotton event MON531 and the cotton host genome |
Validation: |
ring trial validation |
Standardisation: |
EU reference method |
Related Methods: |
QT-TAX-GH-015 |
Type: |
event-specific |
Target GMO name: |
MON531 |
-
Oligonucleotides:
Forward Primer
Name:
531-FSequence:
AACCAATGCCACCCCACTGASize:
20
Reverse Primer
Name:
531-RSequence:
TCCCATTCGAGTTTCTCACGTSize:
21
Probe
Name:
531-PSequence:
TTGTCCCTCCACTTCTTCTCSize:
20
-
Amplicon:
Sequence:
aaccaatgccaccccactgannnnnnnnnnngagaagaagtggagggacaaacgtgagaaactcgaatgggaSize:
72
Documents
Citation | Type | Local copy |
---|---|---|
EU-RL GMFF GMOMETHODS database; QT-EVE-GH-004 | EU reference method protocol |