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Detection Image Detection method details

Name:

 QL-EVE-EC-001

Description:

 Qualitative real-time PCR (TaqMan) method for the detection of event PT73 (TM) (E. coli K-12 event AG3139) (EU-RL GMFF GMOMETHODS database as of November 24, 2017)

Comment:

 Target is the 5' integration border region (IBR) between the insert of event PT73 (TM) and the bacterial host genome

Validation:

 ring trial validation

Standardisation:

 EU reference method

Related Methods:

Type:

 event-specific

Target GMO name:

 PT73 E. coli (TM)

  • Oligonucleotides:

  • Forward Primer

  • Name:

    TMD-For

  • Sequence:

    AATACCGTTAAACGTAAATTCTTTTTCTTT

  • Size:

    30

  • Reverse Primer

  • Name:

    TMD-Rev

  • Sequence:

    TCCTCCCGGTTTTTTTCGTA

  • Size:

    20

  • Probe

  • Name:

    TMD-probe

  • Sequence:

    AGATCGAGTATTCATTCGGTGTATTGATTCACTTGA

  • Size:

    36

  • Amplicon:

  • Sequence:

    aataccgttaaacgtaaattctttttctttnnagatcgagtattcattcggtgtattgattcacttganntacgaaaaaaaccgggagga

  • Size:

    90

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