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Detection Image Detection method details

Name:

 QL-TAX-SS-AM5F/AM5R

Description:

 Quanlitative real-time PCR (TaqMan) method for detection of Salmo salar (Atlantic salmon) (Soga et al. 2020)

Comment:

 Target is the endogenous Growth hormone gene 1 (GH1) of Salmo salar.

The amplicon size according to Soga et al. ((2020) Food Chemistry 305:125426) is not correct. It should be 177 instead of 176 bp.

Validation:

 in-house validation

Standardisation:

 unknown

Related Methods:

Type:

 taxon-specific

Target Species name:

 Salmo salar

  • Oligonucleotides:

  • Forward Primer

  • Name:

    AM5F

  • Sequence:

    AAGGTGCAAAACCATGTTGCCTTCT

  • Size:

    25

  • Reverse Primer

  • Name:

    AM5R

  • Sequence:

    ATGTGAGTGTTCTAGGTCACTAGAC

  • Size:

    25

  • Probe

  • Name:

    AM5PR-2

  • Sequence:

    TGCGTTTCTTGTGCTCTCTATTGCAAAGTA

  • Size:

    30

  • Amplicon:

  • Sequence:

    aaggtgcaaaaccatgttgccttctatttcatgtgccttcctatattttctacagtgcgtttcttgtgctctctattgcaaagtatctttgggtctttaacccatatattattactattattgttcattgatcaagactgttctcgagaaaggtctagtgacctagaacactcacat

  • Size:

    177

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Citation Type Local copy