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Detection method details
Name: |
QL-ELE-AgroBS1 F/AgroBS1 R |
Description: |
Qualitative real-time PCR (TaqMan) method for detection of the Agrobacterium tumefaciens Ti plasmids of the nopaline type aka Agroborder I.
Part of the qualitative triplex real-time PCR (TaqMan) method for detection of Ti plasmid (AgroBorder) sequences and a sequence of the Cassava vein mosaic virus promoter pat construct (P-CsVMV-pat) for screening for components of genetically modified organisms in food (Official Collection of Methods according to § 64 LFGB, L 00.00-176) |
Comment: |
Target are sequences from Agrobacterium tumefaciens Ti plasmids of the nopaline type, which occurs as promoter in genetic engineering constructs and more frequently as construct flanking sequence in various genetically modified plants. |
Validation: |
ring trial validation |
Standardisation: |
national standard |
Related Methods: |
Type: |
element-specific |
Target DNA element: |
V-Flanking Ti-nos-RHIRD
|
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Oligonucleotides:
Forward Primer
Name:
AgroBS1 FSequence:
TGATCATGAGCGGAGAATTAAGGGSize:
24
Reverse Primer
Name:
AgroBS1 RSequence:
CCAAACGTAAAACGGCTTGTCCSize:
22
Probe
Name:
AgroBS1Sequence:
CGTTATGACCCCCGCCGATGACGCSize:
24
-
Amplicon:
Sequence:
tgatcatgagcggagaattaagggagtcacgttatgacccccgccgatgacgcgggacaagccgttttacgtttggSize:
76