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Detection Image Detection method details

Name:

 QL-EVE-16-0-1RB-F/16-0-1RB-R

Description:

 Qualitative real-time PCR (TaqMan) method for detection of papaya event 16-0-1 (Prins at al., 2016)

Comment:

 Target is the 3' integration border region (IBR) between the insert of papaya event 16-0-1 and the papaya host genome.

In Prins et al. (2016), the reverse primer was erroneously indicated as 16-0-1RB-F instead of 16-0-1RB-R. The nucleotide sequences of the 249 bp spanning the Right Border of event 16-0-1 is given in GenBank KU376441.

Validation:

 unknown

Standardisation:

 none

Related Methods:

Type:

 event-specific

Target GMO name:

 16-0-1

  • Oligonucleotides:

  • Forward Primer

  • Name:

    16-0-1RB-F

  • Sequence:

    TGCCTATTTTGAAATTTGGATAC

  • Size:

    23

  • Reverse Primer

  • Name:

    16-0-1RB-R

  • Sequence:

    TCTCCGCTCATGATCAGA

  • Size:

    18

  • Probe

  • Name:

    16-0-1RB-P

  • Sequence:

    TGTCGTTTCCCGCCTTCAGT

  • Size:

    20

  • Amplicon:

  • Sequence:

    TGCCTATTTTGAAATTTGGATACTATCTCATTCAACACTGATAGTTTAAACTGAAGGCGGGAAACGACAATCTGATCATGAGCGGAGA

  • Size:

    88

Documents

Citation Type Local copy
Prins, T.W., Scholtens, I.M.J, Bak, A.W., van Dijk, J.P., Voorhuijzen, M.M., Laurensse, E.J., Kok, E.J. (2016). A case study to determine the geographical origin of unknown GM papaya in routine food sample analysis, followed by identification of papaya events 16-0-1 and 18-2-4. Food Chemistry, 213, 536–544.Link to the document url. peer-reviewed article