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Detection Image Detection method details

Name:

 QT-EVE-ZM-025

Description:

 Quantitative PCR method for detection of maize event MON 87403 (EURL GMFF, GMOMETHODS database, as of May 09, 2018)

Comment:

 Target is the 3' integration border region (IBR) between the insert of maize event MON87403 and the maize host genome.

Validation:

 ring trial validation

Standardisation:

 EU reference method

Related Methods:

 QT-TAX-ZM-002

Type:

 event-specific

Target GMO name:

 MON87403

  • Oligonucleotides:

  • Forward Primer

  • Name:

    87403 QF

  • Sequence:

    CTTTCTTTTTCTCCATATTGACCATCATAC

  • Size:

    30

  • Reverse Primer

  • Name:

    87403 QR

  • Sequence:

    TACTCCGGAATGAGTGCTCTGTATC

  • Size:

    25

  • Probe

  • Name:

    87403 QP

  • Sequence:

    TCATTGCGATCCACATTTCCCTACATGG

  • Size:

    28

  • Amplicon:

  • Sequence:

    ctttcttttt ctccatattg accatcatac tcattgcgat ccacatttcc ctacatggnn nnngatacag agcactcatt ccggagta

  • Size:

    88

Documents

Citation Type Local copy
EU-RL GMFF GMOMETHODS database; QT-EVE-ZM-025Link to the document url. EU reference method protocol