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Detection Image Detection method details

Name:

 QT-EVE-BN-003

Description:

 Quantitative real-time PCR (TaqMan) method for detection of oilseed rape event Rf3 (EU-RL GMFF GMOMETHODS database as of October 01, 2014)

Comment:

 Target is the 3' integration border region (IBR) between the insert of oilseed rape event Rf3 and the oilseed rape host genome

Validation:

 ring trial validation

Standardisation:

 EU reference method

Related Methods:

 QT-TAX-BN-012

Type:

 event-specific

Target GMO name:

 Rf3

  • Oligonucleotides:

  • Forward Primer

  • Name:

    DPA165

  • Sequence:

    CATAAAGGAAGATGGAGACTTGAG

  • Size:

    24

  • Reverse Primer

  • Name:

    KVM084

  • Sequence:

    AGCATTTAGCATGTACCATCAGACA

  • Size:

    25

  • Probe

  • Name:

    TM010

  • Sequence:

    CGCACGCTTATCGACCATAAGCCCA

  • Size:

    25

  • Amplicon:

  • Sequence:

    cataaaggaagatggagacttgagnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnntgggcttatggtcgataagcgtgcgnntgtctgatggtacatgctaaatgct

  • Size:

    139

Documents

Citation Type Local copy
EU-RL GMFF GMOMETHODS database; QT-EVE-BN-003Link to the document url. EU reference method protocol
Savini C., Bogni A., Mazzara M., Van Den Eede G.; Event-specific Method for the Quantification of Oilseed Rape Line Rf3 Using Real-time PCR - Validation Report and Protocol - Seeds Sampling and DNA Extraction Online Publication (2007)Link to the document url. EU reference method protocol