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Detection Image Detection method details

Name:

 QL-ELE-Cry1Ac-F(/R)-n4/Cry1AcR-n2

Description:

 Qualitative real-time PCR (TaqMan) method for detection of the cry1Ac gene (Scholtens et al., 2013).

Comment:

 Target is the cry1Ac gene from bacterium Bacillus thuringiensis.

Validation:

 in-house validation

Standardisation:

 none

Related Methods:

Type:

 element-specific

Target DNA element:
CS-cry1Ac-BACTU

  • Oligonucleotides:

  • Forward Primer

  • Name:

    Cry1Ac-F(/R)-n4

  • Sequence:

    TTCAGGACCAGGATTCAC

  • Size:

    18

  • Reverse Primer

  • Name:

    Cry1AcR-n2

  • Sequence:

    GTGAATAGGGGTCACAGAAGCATA

  • Size:

    24

  • Probe

  • Name:

    Cry1AcP-n3

  • Sequence:

    TCTGGTAGATGTGGATGGGAAGT

  • Size:

    23

  • Amplicon:

  • Sequence:

    ttcaggaccaggattcactggtggagacctcgttagactcaacagcagtggaaacaacattcagaatagagggtatattgaagttccaattcacttcccatccacatctaccagatatagagttcgtgtgaggtatgcttctgtgacccctattcac

  • Size:

    157

Documents

Citation Type Local copy
Xu J, Miao H, Wu H, Huang W, R. Tang R, Qiu M, Wen J, Zhu S, Li Y (2006) Screening genetically modified organisms using multiplex-PCR coupled with oligonucleotide microarray. Biosensors and Bioelectronics 22, 71-77 doi: 10-1016/j.bios.2005.12.001.Link to the document url.
Grisolia, C. K., Oliveira, R., Domingues, I., Oliveira-Filho, E. C., Monerat, R. G., & Soares, A. M. V. M. (2009). Genotoxic evaluation of different delta-endotoxins from Bacillus thuringiensis on zebrafish adults and development in early life stages. Mutation Research-Genetic Toxicology and Environmental Mutagenesis, 672(2), 119-123.Link to the document url. peer-reviewed article