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Detection method details
Name: |
QL-ELE-Cry3Bbf-n2/Cry3Bbr-n2 |
Description: |
Qualitative real-time PCR (TaqMan) method for detection of cry3Bb gene (Scholtens et al., 2013; GMDD). |
Comment: |
Target is the cry3Bb gene from bacterium Bacillus thuringiensis subsp. kumamotoensis. |
Validation: |
in-house validation |
Standardisation: |
none |
Related Methods: |
Type: |
element-specific |
Target DNA element: |
CS-cry3Bb1-BACUK
|
-
Oligonucleotides:
Forward Primer
Name:
Cry3Bbf-n2Sequence:
CCGCCCAGGACTCCATCGSize:
18
Reverse Primer
Name:
Cry3Bbr-n2Sequence:
GAGGCACCCGAGGACAGGSize:
18
Probe
Name:
Cry3BbP-n3Sequence:
CTGCCGCCTGAGACCACTGACGAGCSize:
25
-
Amplicon:
Sequence:
ccgcccaggactccatcgaccagctgccgcctgagaccactgacgagcccctggagaaggcctactcccaccagctgaactacgcggagtgcttcctgatgcaagaccgcaggggcaccatccccttcttcacctggacccaccgctccgtcgacttcttcaacaccatcgacgccgagaagatcacccagctgcccgtggtcaaggcctacgccctgtcctcgggtgcctcSize:
232
Documents
Citation | Type | Local copy |
---|---|---|
Scholtens et al. (2012) |