Detection method details
Name: |
QT-CON-00-006 |
Description: |
Quantitative real-time PCR (TaqMan) method for detection of maize event Bt11 (Shindo et al., 2002) |
Comment: |
Target is the junction region between the Intron 6 (IVS6) from maize alcohol dehydrogenase 1 gene (adh1-1S) and a synthetic cryIA(b) gene |
Validation: |
ring trial validation |
Standardisation: |
ISO standard |
Related Methods: |
QT-TAX-ZM-006 |
Type: |
construct-specific |
Target GMO names: |
Bt10 maize, Bt11
|
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Oligonucleotides:
Forward Primer
Name:
Bt11 3-5’Sequence:
AAAAGACCACAACAAGCCGCSize:
20
Reverse Primer
Name:
Bt11 3-3'Sequence:
CAATGCGTTCTCCACCAAGTACTSize:
23
Probe
Name:
Bt11-2-TaqSequence:
CGACCATGGACAACAACCCAAACATCASize:
27
-
Amplicon:
Sequence:
aaaagaccacaacaagccgcnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnagtacttggtggagaacgcattgSize:
127
Documents
| Citation | Type | Local copy |
|---|---|---|