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Detection Image Detection method details

Name:

 QT-CON-00-007

Description:

 Quantitative real-time PCR (TaqMan) method for detection of maize event Bt176 (Shindo et al., 2002)

Comment:

 Target is the junction region between a synthetic cryIA(b) gene and the phospho-enol-pyruvate carboxylase (PEPC) intron N.9

Validation:

 ring trial validation

Standardisation:

 ISO standard

Related Methods:

 QT-TAX-ZM-006

Type:

 construct-specific

Target GMO names:
B827, Bt176

  • Oligonucleotides:

  • Forward Primer

  • Name:

    E176 2-5'

  • Sequence:

    TGTTCACCAGCAGCAACCAG

  • Size:

    20

  • Reverse Primer

  • Name:

    E176 2-3'

  • Sequence:

    ACTCCACTTTGTGCAGAACAGATCT

  • Size:

    25

  • Probe

  • Name:

    E176-Taq

  • Sequence:

    CCGACGTGACCGACTACCACATCGA

  • Size:

    25

  • Amplicon:

  • Sequence:

    tgttcaccagcagcaaccagnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnagatctgttctgcacaaagtggagt

  • Size:

    100

Documents

Citation Type Local copy
EU-RL GMFF GMOMETHODS database; QT-CON-00-007Link to the document url. EU reference method protocol
Shindo Y., Kuribara H., Matsuoka T., Futo S., Sawada C., Shono J., Akiyama H., Goda Y., Toyoda M., Hino A.; Validation of real-time PCR analyses for line-specific quantitation of genetically modified maize and soybean using new reference molecules J AOALink to the document url. peer-reviewed article
Foodstuffs - Methods of analysis for the detection of genetically modified organisms and derived products - Quantitative nucleic acid based methods ISO 21570:1-103 (2005)Link to the document url. other document