Detection method details
Name: |
QT-TAX-BN-003 |
Description: |
Quantitative real-time PCR (TaqMan) method for detection of oilseed rape cruciferin A gene (Jacchia et al. 2019) (EU-RL GMFF GMOMETHODS database as of January 15, 2020) |
Comment: |
Target is the cruciferin A (cruA) gene in oilseed rape (Brassica napus) |
Validation: |
ring trial validation |
Standardisation: |
EU reference method |
Related Methods: |
Type: |
taxon-specific |
Target Species name: |
Brassica napus |
-
Oligonucleotides:
Forward Primer
Name:
MDB510Sequence:
GGCCAGGGTTTCCGTGATSize:
18
Reverse Primer
Name:
MDB511Sequence:
CCGTCGTTGTAGAACCATTGGSize:
21
Probe
Name:
TM458Sequence:
AGTCCTTATGTGCTCCACTTTCTGGTGCASize:
29
-
Amplicon:
Sequence:
ggccagggtttccgtgatntgcaccagaaagtggagcacataaggactnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnccaatggttctacaacgacggSize:
101
Documents
Citation | Type | Local copy |
---|---|---|
EU-RL GMFF GMOMETHODS database; QT-TAX-BN-003 | EU reference method protocol |