Detection method details
Name: |
QT-CON-00-003 |
Description: |
Quantitative real-time PCR (TaqMan) method for detection of soybean event GTS 40-3-2 (ISO/FDIS 21570). |
Comment: |
Target is the junction region between the Cauliflower mosaic virus 35S promoter (CaMV P-35S) and the chloroplast transit peptide sequence from Petunia hybrida epsps gene (ctp4). |
Validation: |
ring trial validation |
Standardisation: |
ISO standard |
Related Methods: |
QT-TAX-GM-002 |
Type: |
construct-specific |
Target GMO names: |
GTS 40-3-2
|
-
Oligonucleotides:
Forward Primer
Name:
RR1-FSequence:
CATTTGGAGAGGACACGCTGASize:
21
Reverse Primer
Name:
RR1-RSequence:
GAGCCATGTTGTTAATTTGTGCCSize:
23
Probe
Name:
RR1Sequence:
CAAGCTGACTCTAGCAGATCTTTCSize:
24
-
Amplicon:
Sequence:
catttggagaggacacgctgacaagctgactctagcagatctttcnnnnnnggcacaaattaacaacatggctcSize:
74
Documents
Citation | Type | Local copy |
---|---|---|
EU-RL GMFF GMOMETHODS database; QT-CON-00-003 | EU reference method protocol | |
Foodstuffs - Methods of analysis for the detection of genetically modified organisms and derived products - Quantitative nucleic acid based methods ISO 21570:1-103 (2005) | other document |