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Detection Image Detection method details

Name:

 QT-TAX-OS-003

Description:

 Quantitative real-time PCR (TaqMan) method for detection of rice sucrose-phosphate synthase gene (Jiang et al., 2009)

Comment:

 Target is the endogenous sucrose-phosphate synthase (SPS) gene of Oryza sativa.

BLAST analysis against GenBank (February 2024) shows two mismatches between the sequence of the forward primer and 12 of the 14 listed rice sequences (including the rice reference genome Nipponbare). First analyses performed by WFSR indicated that an adaptation of the forward primer sequence leads to an increased sensitivity in the detection of several rice varieties.

Validation:

 ring trial validation

Standardisation:

 unknown

Related Methods:

Type:

 taxon-specific

Target Species name:

 Oryza sativa

  • Oligonucleotides:

  • Forward Primer

  • Name:

    SPS-1F

  • Sequence:

    TTGCGCCTGAACGGATAT

  • Size:

    18

  • Reverse Primer

  • Name:

    SPS-3R

  • Sequence:

    CGGTTGATCTTTTCGGGATG

  • Size:

    20

  • Probe

  • Name:

    SPS-P

  • Sequence:

    TCCGAGCCGTCCGTGCGTC

  • Size:

    19

  • Amplicon:

  • Sequence:

    ttgcgcctgaacggatatnnnnnnnnnnnnnnnnnnnnnnngacgcacggacggctcggancatcccgaaaagatcaaccg

  • Size:

    81

Documents

Citation Type Local copy
Jiang L., Yang L., Zhang H., Guo J., Mazzara M., Van den Eede G., Zhang D.; International collaborative study of the endogenous reference gene, sucrose phosphate synthase (SPS), used for qualitative and quantitative analysis of genetically modified riceLink to the document url. peer-reviewed article
EU-RL GMFF GMOMETHODS database; QT-TAX-OS-003Link to the document url. EU reference method protocol