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Detection Image Detection method details

Name:

 QT-TAX-CP-001

Description:

 Quantitative real-time (TaqMan) PCR method for detection of papaya chymopapain (chy) gene (EU-RL GMFF GMOMETHODS database as of September 13, 2016)

Comment:

 Target is the endogenous chymopapain (chy) gene from papaya (Carica papaya)

Validation:

 ring trial validation

Standardisation:

 EU reference method

Related Methods:

Type:

 taxon-specific

Target Species name:

 Carica papaya

  • Oligonucleotides:

  • Forward Primer

  • Name:

    Q-Chy-1F

  • Sequence:

    CCATGCGGATCCTCCCA

  • Size:

    17

  • Reverse Primer

  • Name:

    Q-Chy-2R

  • Sequence:

    CATCGTAGCCATTGTAACACTAGCTAA

  • Size:

    27

  • Probe

  • Name:

    Q-Chy-P

  • Sequence:

    TTCCCTTCATCCATTCCCACTCTTGAGA

  • Size:

    28

  • Amplicon:

  • Sequence:

    ccatgcggatcctcccatttcccttcatccattcccactcttgagagttagctagtgttacaatggctacgatg

  • Size:

    74

Documents

Citation Type Local copy
Guo, J. C., Yang, L. T., Liu, X., Guan, X. Y., Jiang, L. X., & Zhang, D. B. (2009). Characterization of the exogenous insert and development of event-specific PCR detection methods for genetically modified Huanong no. 1 papaya. Journal of Agricultural and Food Chemistry, 57(16), 7205-7212.Link to the document url. peer-reviewed article
Wei, J. et al. (2016). Collaborative trial for the validation of event-specific PCR detection methods of genetically modified papaya Huanong No. 1. Food Chemistry, 194, 20-25.Link to the document url. peer-reviewed article
EU-RL GMFF GMOMETHODS database; QT-TAX-CP-001Link to the document url. EU reference method protocol