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Detection Image Detection method details

Name:

 QT-EVE-ZM-021

Description:

 Quantitative real-time PCR (TaqMan) method for detection of maize event 98140 (EU-RL GMFF GMOMETHODS database as January 10, 2014)

Comment:

 Target is the 5' integration border region (IBR) between the insert of maize event 98140 and the maize host genome

Validation:

 ring trial validation

Standardisation:

 EU reference method

Related Methods:

 QT-TAX-ZM-014

Type:

 event-specific

Target GMO name:

 98140

  • Oligonucleotides:

  • Forward Primer

  • Name:

    DP098-f6

  • Sequence:

    GTGTGTATGTCTCTTTGCTTGGTCTT

  • Size:

    26

  • Reverse Primer

  • Name:

    DP098-r2

  • Sequence:

    GATTGTCGTTTCCCGCCTTC

  • Size:

    20

  • Probe

  • Name:

    DP098-p5

  • Sequence:

    CTCTATCGATCCCCCTCTTTGATAGTTTAAACT

  • Size:

    33

  • Amplicon:

  • Sequence:

    gtgtgtatgtctctttgcttggtcttnctctatcgatccccctctttgatagtttaaactgaaggcgggaaacgacaatc

  • Size:

    80

Documents

Citation Type Local copy
Savini C., Bonfini L., Querci M., Mazzara M., Cordeil S., Van Den Eede G.; Event-specific Method for the Quantification of Maize 98140 Using Real-time PCR - Validation Report and Protocol Online Publication (2011)Link to the document url. EU reference method protocol
EU-RL GMFF GMOMETHODS database; QT-EVE-ZM-021Link to the document url. EU reference method protocol