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Detection Image Detection method details

Name:

 QT-EVE-GH-008

Description:

 Quantitative real-time PCR (TaqMan) method for detection of cotton event GHB119 (EU-RL GMFF GMOMETHODS database as of July 21, 2014).

Comment:

 Target is the 3' integration border region (IBR) between the insert of cotton event GHB119 and the cotton host genome.

Validation:

 ring trial validation

Standardisation:

 EU reference method

Related Methods:

Type:

 event-specific

Target GMO name:

 GHB119

  • Oligonucleotides:

  • Forward Primer

  • Name:

    SHA021

  • Sequence:

    CCAGTACTAAAATCCAGATCATGCA

  • Size:

    25

  • Reverse Primer

  • Name:

    NEL109

  • Sequence:

    GAAATTGCGTGACTCAAATTCC

  • Size:

    22

  • Probe

  • Name:

    TM082

  • Sequence:

    CCTGCAGGTCGACGGCCGAGTAC

  • Size:

    23

  • Amplicon:

  • Sequence:

    ccagtactaaaatccagatcatgcannnncctgcaggtcgacggccgagtacnnnnnnnnnnnnnnnnggaatttgagtcacgcaatttc

  • Size:

    90

Documents

Citation Type Local copy
EU-RL GMFF GMOMETHODS database; QT-EVE-GH-008Link to the document url. EU reference method protocol