Help icon

Detection Image Detection method details

Name:

 QL-PLN-00-007

Description:

 Qualitative conventional PCR method for detection of chloroplast tRNA-Leu intron (ISO/FDIS 21569)

Comment:

 Target is the conserved chloroplast tRNA-Leu (trnL) intron. For different plant species the amplicon size varies between approx. 500-600 bp. The amplicon shown here is of a potato sample.

Validation:

 ring trial validation

Standardisation:

 ISO standard

Related Methods:

Type:

 taxon-specific

Target Species name:

 all-plants

  • Oligonucleotides:

  • Forward Primer

  • Name:

    A1

  • Sequence:

    CGAAATCGGTAGACGCTACG

  • Size:

    20

  • Reverse Primer

  • Name:

    A2

  • Sequence:

    GGGGATAGAGGGACTTGAAC

  • Size:

    20

  • Amplicon:

  • Sequence:

    cgaaatcggtagacgctacgnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnngttcaagtccctctatcccc

  • Size:

    571

Documents

Citation Type Local copy
Foodstuffs - Methods of analysis for the detection of genetically modified organisms and derived products - Qualitative nucleic acid based methods ISO 21569:1-69 (2005)Link to the document url. other document
EU-RL GMFF GMOMETHODS database; QL-PLN-00-007Link to the document url. EU reference method protocol