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Detection Image Detection method details

Name:

 QL-EVE-MS-J163-F/J163-R

Description:

 Qualitative real-time PCR (TaqMan) method for detection of alfalfa event J163 (Guertler et al. 2018).

Comment:

 Target is the 5' integration border region (IBR) between the insert of alfalfa event J163 and the alfalfa host genome.

Validation:

 ring trial validation

Standardisation:

 national standard

Related Methods:

 QL-TAX-Acc1-F/Acc1-R

Type:

 event-specific

Target GMO name:

 J163

  • Oligonucleotides:

  • Forward Primer

  • Name:

    J163-F

  • Sequence:

    CGGGACAAGGTCATCCAAACTG

  • Size:

    22

  • Reverse Primer

  • Name:

    J163-R

  • Sequence:

    ACCTTGTTGAGGCTTTGGACTG

  • Size:

    22

  • Probe

  • Name:

    J163-P

  • Sequence:

    TCTGCAGGTCCTGCTCGAGTGGAAGCT

  • Size:

    27

  • Amplicon:

  • Sequence:

    CGGGACAAGGTCATCCAAACTGAAGTGTTCGGTGGGAAACGACACTCTGATCCCCATCAAGCTTCTGCAGGTCCTGCTCGAGTGGAAGCTAATTCTCAGTCCAAAGCCTCAACAAGGT

  • Size:

    118

Documents

Citation Type Local copy
Guertler et al., Development of event-specific qPCR detection methods for genetically modified alfalfa events J101, J163 and KK179. Biomolecular Detection and Quantification 2019, 17, 100076.Link to the document url. peer-reviewed article