Detection method details
Name: |
QT-TAX-OS-003 |
Description: |
Quantitative real-time PCR (TaqMan) method for detection of rice sucrose-phosphate synthase gene (Jiang et al., 2009) |
Comment: |
Target is the endogenous sucrose-phosphate synthase (SPS) gene of Oryza sativa |
Validation: |
ring trial validation |
Standardisation: |
unknown |
Related Methods: |
Type: |
taxon-specific |
Target Species name: |
Oryza sativa |
-
Oligonucleotides:
Forward Primer
Name:
SPS-1FSequence:
TTGCGCCTGAACGGATATSize:
18
Reverse Primer
Name:
SPS-3RSequence:
CGGTTGATCTTTTCGGGATGSize:
20
Probe
Name:
SPS-PSequence:
TCCGAGCCGTCCGTGCGTCSize:
19
-
Amplicon:
Sequence:
ttgcgcctgaacggatatnnnnnnnnnnnnnnnnnnnnnnngacgcacggacggctcggancatcccgaaaagatcaaccgSize:
81
Documents
Citation | Type | Local copy |
---|---|---|
Jiang L., Yang L., Zhang H., Guo J., Mazzara M., Van den Eede G., Zhang D.; International collaborative study of the endogenous reference gene, sucrose phosphate synthase (SPS), used for qualitative and quantitative analysis of genetically modified rice | peer-reviewed article | |
EU-RL GMFF GMOMETHODS database; QT-TAX-OS-003 | EU reference method protocol |