Detection method details
Name: |
QL-ELE-npt 1-5'/npt 1-3' |
Description: |
Qualitative real-time PCR (TaqMan) method for detection of the nptII gene (Scholtens et al., 2013). |
Comment: |
Target is the neomycin phosphotransferase II (nptII) gene from bacterium Escherichia coli. |
Validation: |
in-house validation |
Standardisation: |
none |
Related Methods: |
Type: |
element-specific |
Target DNA element: |
CS-nptII-ECOLX, V-nptII-ECOLX
|
-
Oligonucleotides:
Forward Primer
Name:
npt 1-5'Sequence:
GACAGGTCGGTCTTGACAAAAAGSize:
23
Reverse Primer
Name:
npt 1-3'Sequence:
GAACAAGATGGATTGCACGCSize:
20
Probe
Name:
nptII-probeSequence:
TGCCCAGTCATAGCCGAATAGCCTCTCCASize:
29
-
Amplicon:
Sequence:
gacaggtcggtcttgacaaaaagaaccgggcgcccctgcgctgacagccggaacacggcggcatcagagcagccgattgtctgttgtgcccagtcatagccgaatagcctctccacccaagcggccggagaacctgcgtgcaatccatcttgttcSize:
155