Detection method details
Name: |
QT-TAX-GH-015 |
Description: |
Quantitative real-time PCR (TaqMan) method for detection of cotton fiber-specific acyl carrier protein (ACP1) gene (EU-RL GMFF GMOMETHODS database as of January 07, 2014).
Estimated number of target copies per haploid genome (Jacchia et al. 2018): 2 |
Comment: |
Target is the fiber-specific acyl carrier protein (ACP1) gene from Gossypium hirsutum.
This assay is NOT recommended for GMO quantitative analysis because of a secondary target with mismatches in the primers and probe annealing region. |
Validation: |
ring trial validation |
Standardisation: |
EU reference method |
Related Methods: |
Type: |
taxon-specific |
Target Species name: |
Gossypium hirsutum |
-
Oligonucleotides:
Forward Primer
Name:
primer forwardSequence:
ATTGTGATGGGACTTGAGGAAGASize:
23
Reverse Primer
Name:
primer reverseSequence:
CTTGAACAGTTGTGATGGATTGTGSize:
24
Probe
Name:
probeSequence:
ATTGTCCTCTTCCACCGTGATTCCGAASize:
27
-
Amplicon:
Sequence:
attgtgttgggacttgaggaaganttcggaatcacggtggaagaggacaacncacaatccatcacaactgttcaagSize:
76
Documents
Citation | Type | Local copy |
---|---|---|
Jacchia S., Kagkli D.F., Lievens A., Angers-Loustau A., Savini C., Emons H., Mazzara M. (2018) Identification of single target taxon-specific reference assays for the most commonly genetically transformed crops using digital droplet PCR. Food Control 93,191-200 | peer-reviewed article | |
EU-RL GMFF GMOMETHODS database; QT-TAX-GH-015 | EU reference method protocol |